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Journal: Chinese Herbal Medicines
Article Title: Astragalus polysaccharides inhibit arsenic trioxide-induced BMSCs damage through inhibition of Jnk and p38 signaling pathways
doi: 10.1016/j.chmed.2025.03.007
Figure Lengend Snippet: APS modulates apoptotic protein expressions in BMSCs exposed to ATO. Cells were exposed to 0.5 μmol/L ATO, and 0.5 μmol/L ATO + APS (20, 40, 100, and 200 μg/mL) for 48 h. (A) Western blotting analysis was applied to detect protein expression of p16, p53, cleaved Caspase-3, Caspase-3, Bcl-2 and Bax, β -actin was used as a loading control, n = 3. (B) Western blotting analysis was used to detect protein expression of p-Akt and Akt, β -actin was served as a loading control, n = 3. A two-tailed Student’s t -test was used to compare differences between two experimental groups. Error bars represent mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control group, # P < 0.05, ## P < 0.01 vs ATO group.
Article Snippet: The membranes were then incubated with primary antibodies against B-cell lymphoma 2 (Bcl-2) (1:1 000, Proteintech, Chicago, USA, Cat. No. 68103-1-lg),
Techniques: Western Blot, Expressing, Control, Two Tailed Test